Targeting non coding rnas in plants with the crispr cas technology is a challenge yet worth accepting knockdown or knockout of ncrna genes compared to protein coding genes using the crispr cas technology is a challenge rna exosome regulated long non coding rna transcription controls super enhancer activity. Introduction as a class of rnas that have no or little protein coding potential the mechanism underlying the functions of non protein coding rnas ncrnas or npcrnas is a fascinating area of research the recent wide applications of the high throughput rna sequencing rna seq approaches have facilitated the identification of thousands of novel ncrnas or npcrnas in many organisms such as . Non coding rnas ncrnas that is rnas not translated into proteins are crucial regulators of a variety of biological processes in plants while protein encoding genes have been relatively well . Non coding rnas plant sciences the discovery of peptides encoded by what were thought to be non coding or junk regions of precursors to microrna sequences reveals a new layer of gene . Non coding rnas ncrnas have emerged as critical components of gene regulatory networks across a plethora of plant species in particular the 20 30 nucleotide small non coding rnas srnas play important roles in mediating both developmental processes and responses to biotic stresses based on variation in their biogenesis pathways a number of different srna classes have been identified
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